Lesson 3.1.5.2.1

3.1.5.2.1 Semi-conservative DNA replication Quiz: AQA Biology, Unit 1

20 questions

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Lesson 3.1.5.2.1, Semi-conservative DNA replication: 20 multiple choice questions for the AQA Biology (7402), Unit 1: Biological molecules, written with Revision Ninja.

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The 20 questions

  1. Which enzyme unwinds the DNA double helix and breaks the hydrogen bonds between bases during replication?

    • ATP hydrolase
    • ATP synthase
    • DNA polymerase
    • DNA helicase
  2. Which enzyme joins adjacent nucleotides together during DNA replication?

    • Lipase
    • Amylase
    • DNA helicase
    • DNA polymerase
  3. How many original strands are present in each daughter DNA double helix?

    • None
    • Four
    • One
    • Two
  4. Which bonds break when DNA unwinds during replication?

    • Ionic bonds
    • Ester bonds
    • Phosphodiester bonds
    • Hydrogen bonds
  5. How does a template DNA strand determine the sequence of a new strand?

    • Phosphodiester bond formation
    • Hydrogen bond hydrolysis
    • Complementary base pairing
    • Active transport
  6. Which bond is formed by DNA polymerase during DNA replication?

    • Hydrogen bond
    • Peptide bond
    • Glycosidic bond
    • Phosphodiester bond
  7. Which scientists provided experimental evidence for semi-conservative DNA replication using nitrogen isotopes?

    • Watson and Crick
    • Meselson and Stahl
    • Rosalind Franklin
    • Hershey and Chase
  8. A DNA molecule has 1000 base pairs and replicates once. How many new nucleotides must be assembled in total?

    • 2000
    • 500
    • 4000
    • 1000
  9. A DNA molecule labelled with heavy nitrogen (15N) is replicated for two generations in light nitrogen (14N). What fraction of the resulting DNA molecules contain only 14N?

    • 0
    • 1/2
    • 3/4
    • 1/4
  10. A DNA molecule replicates three times. How many DNA molecules are produced from one starting molecule?

    • 3
    • 6
    • 8
    • 16
  11. What is the immediate effect of inhibiting DNA helicase during replication?

    • Primers cannot bind
    • Backbone breaks apart
    • Strands cannot separate
    • Ribosomes stop working
  12. During replication of a DNA molecule with 100 A-T base pairs, how many hydrogen bonds must be broken to separate the strands?

    • 200
    • 300
    • 400
    • 100
  13. What acts as the template during the synthesis of a new DNA strand?

    • An original strand
    • A new strand
    • A tRNA molecule
    • An mRNA strand
  14. In which direction does DNA polymerase synthesise a new DNA strand?

    • 3' to 1' direction
    • 5' to 3' direction
    • 5' to 2' direction
    • 3' to 5' direction
  15. In the Meselson and Stahl experiment, what band was observed after one generation in light nitrogen?

    • One intermediate band
    • One heavy band
    • Two separate bands
    • One light band
  16. A DNA molecule of 500 base pairs (1000 nucleotides in total) replicates four times in a medium containing all required nucleotides. How many new nucleotides are assembled overall?

    • 14000
    • 15000
    • 16000
    • 1000
  17. Why does semi-conservative replication ensure genetic continuity between cell generations?

    • Causes frequent mutations
    • Synthesises triple helices
    • Preserves original sequence
    • Produces random bases
  18. Which nitrogenous base always pairs with adenine in DNA?

    • Cytosine
    • Guanine
    • Thymine
    • Uracil
  19. Why were nitrogen isotopes used in the Meselson and Stahl experiment?

    • Different chemical charges
    • Different fluorescence levels
    • Different radioactivities
    • Different molecular densities
  20. What can prevent a daughter DNA molecule from being genetically identical to its parent?

    • Replication errors
    • Helicase action
    • Phosphodiester bonds
    • Hydrogen bonding

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